Diagnostic Immunology and Flow Cytometry Topic Center

Serving as the expert scientific and educational resource for the College of American Pathologists (CAP).

Educational resources on diagnostic immunology and flow cytometry

Immunology-Related Journal Articles

• Laboratory Detection and Initial Diagnosis of Monoclonal Gammopathies: Guidelines From the College of American Pathologists in Collaboration With the American Association for Clinical Chemistry and the American Society for Clinical Pathology
• Variability in the Laboratory Measurement of Cytokines: A Longitudinal Summary of a College of American Pathologists Testing Survey
• Criteria for Diagnosis, Staging, Risk Stratification and Response Assessment of Multiple Myeloma
• Screening and Diagnosis of Monoclonal Gammopathies. An International Survey of Laboratory Practice
• New Insights on the Analytical Performances for Detecting and Quantifying Monoclonal Proteins (Editorial)
• An International Multi-Center Serum Protein Electrophoresis Accuracy and M-Protein Isotyping Study. Part I: Factors Impacting Limit of Quantitation of Serum Protein Electrophoresis.
• An International Multi-Center Serum Protein Electrophoresis Accuracy and M-Protein Isotyping Study. Part II: Limit of Detection and Follow-up of Patients with Small M-Proteins.

Flow Cytometry-Related Journal Articles

• CAP Flow Cytometry Checklist; Difficult and New Items (International Clinical Cytometry Society (ICCS) e-Newsletter, 2015:6(2)) (PDF, 1.4 MB)
• Marked Variability in Reported Minimal Residual Disease Lower Level of Detection of 4 Hematolymphoid Neoplasms
• EuroFlow Standardization of Flow Cytometer Instrument Settings and Immunophenotyping Protocols
• EuroFlow Antibody Panels for Standardized N-dimensional Flow Cytometric Immunophenotyping of Normal, Reactive and Malignant Leukocytes
• Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group (PDF, 655, KB)
• Diagnosing PNH with FLAER and multiparameter flow cytometry (PDF, 1.4 KB)
• Practice parameter for the diagnosis and management of primary immunodeficiency (PDF, 458 KB)
• Establishment and Validation of a Standard Protocol for the Detection of Minimal Residual Disease in B Lineage Childhood Acute Lymphoblastic Leukemia by Flow Cytometry in a Multi-Center Setting (PDF, 171 KB)
• Minimal Residual Disease Quantitation in Acute Myeloid Leukemia (PDF, 175 KB)
• Report of the European Myeloma Network on Multiparametric Flow Cytometry in Multiple Myeloma and Related Disorders (PDF, 192 KB)

Guidelines, Consensus Statements, and Recommendations

• International Myeloma Working Group Guidelines for Serum-Free Light Chain Analysis in Multiple Myeloma and Related Disorders (PDF, 347 KB)
• NCCN guidelines on multiple myeloma (PDF, 301 KB)
• 2006 Bethesda International Consensus Recommendations
  • Flow Cytometric Immunophenotypic Analysis of Hematolymphoid Neoplasia: Medical Indications (The Cytometry Part B: Clinical Cytometry Supplement 72B:S5-S13, 2007)
  • Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry: Optimal Reagents and Reporting for the Flow Cytometric Diagnosis of Hematopoietic Neoplasia (The Cytometry Part B: Clinical Cytometry Supplement 72B:S14-S22, 2007)
  • Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry: Recommendations for Training and Education to Perform Clinical Flow Cytometry (The Cytometry Part B: Clinical Cytometry Supplement 72B:S23-S33, 2007)
• The International Consensus Statement on an update of the Classification Criteria for Definite Antiphospholipid Syndrome [(APS) Miyakis S, Lockshin MD, Atsumi T, et al. JThromb Haemost. 2006;4:295-306)
• Consensus Guidelines on Anti-Beta 2 Glycoprotein I Testing and Reporting (Wong RCW, Favaloro EJ, Adelstein S, et al. Pathology. 2008;40:58-63)
• Update of the Guidelines for Lupus Anticoagulant Detection (Pengo V, Tripodi A, Reber G, et al. J Thromb Haemost. 2009;7:1737-1740)

Clinical and Laboratory Standards Institute Documents

• Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells; Approved Guideline - Second Edition (Clinical and Laboratory Standards Institute, document H43-A2)
• Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline - Second Edition (Clinical and Laboratory Standards Institute, document H42-A2)
• Evaluation Methods and Analytical Performance Characteristics of Immunological Assays for Human Immunoglobulin E (IgE) Antibodies of Defined Allergen Specificities; Approved Guideline (Clinical and Laboratory Standards Institute, document I/LA20-A)
• Quality Assurance of Laboratory Tests for Autoantibodies to Nuclear Antigens: (1) Indirect Fluorescence Assay for Microscopy and (2) Microtiter Enzyme Immunoassay Methods (Clinical and Laboratory Standards Institute, document ILA02-A2)

Join the Diagnostic Immunology and Flow Cytometry Committee

Learn more about our committee activities and how to join the Diagnostic Immunology and Flow Cytometry Committee.